Sources/Clones
Dako (F5D), Pharmingen (5FD) and Santa Cruz (polyclonal).
Fixation/Preparation
F5D is immunoreactive in fixed, paraffin-embedded tissue sections and HIER enhances immunoreactivity.
Background
Myogenin belongs to a family of regulatory proteins essential for muscle development. Studies in mice indicate that myogenin is not required for the initial aspects of myogenesis, including myotome formation and the appearance of myoblasts, but late stages of embryogenesis are more dependent on myogenin (Venuti et al, 1995). Expression of myogenin is restricted to cells of skeletal muscle origin and appears to be inversely related to the degree of cellular differentiation, making it a potentially useful marker for skeletal muscle differentiation in the identification and typing of anaplastic round cell tumors in childhood (Appendix 1.3).
Applications
F5D recognizes an epitope located in the amino acid region 138-158 of the myogenin protein and labels nuclei of the majority of human rhabdomyosarcomas (Folpe et al, 1997). No reactivity is reported with Ewing's sarcoma/peripheral primitive neuroectodermal tumor, neuroblastoma or adult skeletal muscle (Wang et al, 1995).
Comments
Only nuclear staining should be regarded as positive. Clone F5D shows strong reactivity in paraffin sections following HIER. Myogenin may prove to be a better and more sensitive marker of skeletal muscle differentiation in poorly differentiated rhabdomyosarcoma than MyoD1, given that the latter displays non-specific crossreactivity with an unknown cytoplasmic antigen in non-muscle cells and tumors (Wang et al, 1995).
References
•Folpe AL, Patterson K, Gown AM 1997. Antibodies to desmin identify the blastemal component of nephroblastoma. Modern Pathology; 10: 895-900.
•Venuti JM, Morris JH, Vivian JL et al 1995. Myogenin is required for late but not early aspects of myogenesis during mouse development. Journal of Cell Biology; 128: 563-576.
•Wang NP, Marx J, McNutt MA et al 1995. Expression of myogenic regulatory proteins (myogenin and MyoD1) in small blue round cell tumors of childhood. American Journal of Pathology; 147: 1799-1810.
Bibliografia
Manual of diagnostic antibodies for immunohistology / Anthony S.-Y. Leong, Kumarasen Cooper, F. Joel W.-M. Leong.
Dako (F5D), Pharmingen (5FD) and Santa Cruz (polyclonal).
Fixation/Preparation
F5D is immunoreactive in fixed, paraffin-embedded tissue sections and HIER enhances immunoreactivity.
Background
Myogenin belongs to a family of regulatory proteins essential for muscle development. Studies in mice indicate that myogenin is not required for the initial aspects of myogenesis, including myotome formation and the appearance of myoblasts, but late stages of embryogenesis are more dependent on myogenin (Venuti et al, 1995). Expression of myogenin is restricted to cells of skeletal muscle origin and appears to be inversely related to the degree of cellular differentiation, making it a potentially useful marker for skeletal muscle differentiation in the identification and typing of anaplastic round cell tumors in childhood (Appendix 1.3).
Applications
F5D recognizes an epitope located in the amino acid region 138-158 of the myogenin protein and labels nuclei of the majority of human rhabdomyosarcomas (Folpe et al, 1997). No reactivity is reported with Ewing's sarcoma/peripheral primitive neuroectodermal tumor, neuroblastoma or adult skeletal muscle (Wang et al, 1995).
Comments
Only nuclear staining should be regarded as positive. Clone F5D shows strong reactivity in paraffin sections following HIER. Myogenin may prove to be a better and more sensitive marker of skeletal muscle differentiation in poorly differentiated rhabdomyosarcoma than MyoD1, given that the latter displays non-specific crossreactivity with an unknown cytoplasmic antigen in non-muscle cells and tumors (Wang et al, 1995).
References
•Folpe AL, Patterson K, Gown AM 1997. Antibodies to desmin identify the blastemal component of nephroblastoma. Modern Pathology; 10: 895-900.
•Venuti JM, Morris JH, Vivian JL et al 1995. Myogenin is required for late but not early aspects of myogenesis during mouse development. Journal of Cell Biology; 128: 563-576.
•Wang NP, Marx J, McNutt MA et al 1995. Expression of myogenic regulatory proteins (myogenin and MyoD1) in small blue round cell tumors of childhood. American Journal of Pathology; 147: 1799-1810.
Bibliografia
Manual of diagnostic antibodies for immunohistology / Anthony S.-Y. Leong, Kumarasen Cooper, F. Joel W.-M. Leong.
