Sources/Clones
Polyclonal HSV I and HSV II.
Biodesign, Biogenesis, Biogenex, Chemicon, Dako (polyclonal), Fitzgerald, Immunon and Pharmingen.
Monoclonal Antibody
Accurate (A321, M22253A, HP2M222M53A), American Research Products (1697-151, 1589-136, 1645-18), Biodesign (203, 206, 016, 017), Biogenesis (CHA437, 10527, H62), Biogenex (G16, E10, 023A1909, 045A1930B), EY Labs, Fitzgerald (M22254, M22255, M2110155, M2110156) and Seralab (CHA437).
Fixation/Preparation
Both antibodies 302M and 303M are applicable to frozen cryostat sections as well as fixed paraffin-embedded tissue sections. The latter require microwave pretreatment to eliminate non-specific background staining.
Background
The antigens used in the production of these antibodies comprise detergent-solubilized HSV I- and HSV II -infected whole rabbit cornea cells respectively. The 302M antibody reacts with HSV I- specific antigens whilst the 303M antibody reacts with HSV II- specific antigens. Both antibodies react with antigens common for HSV I and II: all major glycoproteins present in the viral envelope and at least one core protein. There is no demonstrable crossreactivity with varicella zoster virus, cytomegalovirus or Epstein-Barr virus.
Applications
Antibodies 302M and 303M detect the presence of HSV I and HSV II, respectively, in tissue sections, e.g. skin and brain. A diffuse intranuclear signal is produced, often coinciding with the ground-glass intranuclear inclusions of HSV. Similar intranuclear inclusions associated with biotin accumulation have been observed in glandular epithelia of gestational endometrium (Shigeo et al, 1993; Sickel & Di Sant'Agnese 1994). Hence, for the unwary any attempt to demonstrate HSV in these biotin inclusions may produce a false-positive immunoreaction, especially when the avidin-biotin immunodetection system is utilized. The recommended use of prewashing with 0.05% free avidin and 0.05% free biotin does not eliminate this crossimmunoreactivity. It is therefore recommended that the PAP or APAAP immunodetection system be used for any HSV immunohistochemical investigation of gestational endometrium (Cooper et al, 1997).
Comments
Biotin-like activity has been observed in thyroid lesions as well (Kashima et al, 1997). Hence, awareness of this interference is crucial to avoid misinterpretation of immunohistochemical investigations, especially with the ABC immunodetection system. Genital lesions with typical multinucleated giant cells with ''ground" glass-intranuclear inclusions should be used as positive control tissue.
References
•Cooper K, Haffajee Z, Taylor L 1997. Comparative analysis of biotin intranuclear inclusions of gestational endometrium using the APAAP, ABC and the PAP immunodetection systems.
•Journal of Clinical Pathology 50: 153-156. gestational endometrium using the APAAP, ABC and the PAP immunodetection systems. Journal of Clinical Pathology 50: 153-156.
•Kashima K, Yokoyama S, Tsutomu Da, Nakayama I, Nickerson PA, Noguchi S 1997. Cytoplasmic biotin-like activity interferes with immunohistochemical analysis of thyroid lesions: a comparison of antigen retrieval methods. Modern Pathology 10: 515-519.
•Shigeo Y, Kenji K, Souichi I, Daa T, Nakamaya I, Moriushi A 1993. Biotin-containing intranuclear inclusions in endometrial glands during gestation and puerperium. American Journal of Clinical Pathology 99: 13-17.
•Sickel JZ, Di Sant' Agnese A 1994. Anomalous immunostaining of `optically clear' nuclei in gestational endometrium. Archives of Pathology and Laboratory Medicine 118: 831-833.
Bibliografía
Manual of diagnostic antibodies for immunohistology / Anthony S.-Y. Leong, Kumarasen Cooper, F. Joel W.-M. Leong.
Polyclonal HSV I and HSV II.
Biodesign, Biogenesis, Biogenex, Chemicon, Dako (polyclonal), Fitzgerald, Immunon and Pharmingen.
Monoclonal Antibody
Accurate (A321, M22253A, HP2M222M53A), American Research Products (1697-151, 1589-136, 1645-18), Biodesign (203, 206, 016, 017), Biogenesis (CHA437, 10527, H62), Biogenex (G16, E10, 023A1909, 045A1930B), EY Labs, Fitzgerald (M22254, M22255, M2110155, M2110156) and Seralab (CHA437).
Fixation/Preparation
Both antibodies 302M and 303M are applicable to frozen cryostat sections as well as fixed paraffin-embedded tissue sections. The latter require microwave pretreatment to eliminate non-specific background staining.
Background
The antigens used in the production of these antibodies comprise detergent-solubilized HSV I- and HSV II -infected whole rabbit cornea cells respectively. The 302M antibody reacts with HSV I- specific antigens whilst the 303M antibody reacts with HSV II- specific antigens. Both antibodies react with antigens common for HSV I and II: all major glycoproteins present in the viral envelope and at least one core protein. There is no demonstrable crossreactivity with varicella zoster virus, cytomegalovirus or Epstein-Barr virus.
Applications
Antibodies 302M and 303M detect the presence of HSV I and HSV II, respectively, in tissue sections, e.g. skin and brain. A diffuse intranuclear signal is produced, often coinciding with the ground-glass intranuclear inclusions of HSV. Similar intranuclear inclusions associated with biotin accumulation have been observed in glandular epithelia of gestational endometrium (Shigeo et al, 1993; Sickel & Di Sant'Agnese 1994). Hence, for the unwary any attempt to demonstrate HSV in these biotin inclusions may produce a false-positive immunoreaction, especially when the avidin-biotin immunodetection system is utilized. The recommended use of prewashing with 0.05% free avidin and 0.05% free biotin does not eliminate this crossimmunoreactivity. It is therefore recommended that the PAP or APAAP immunodetection system be used for any HSV immunohistochemical investigation of gestational endometrium (Cooper et al, 1997).
Comments
Biotin-like activity has been observed in thyroid lesions as well (Kashima et al, 1997). Hence, awareness of this interference is crucial to avoid misinterpretation of immunohistochemical investigations, especially with the ABC immunodetection system. Genital lesions with typical multinucleated giant cells with ''ground" glass-intranuclear inclusions should be used as positive control tissue.
References
•Cooper K, Haffajee Z, Taylor L 1997. Comparative analysis of biotin intranuclear inclusions of gestational endometrium using the APAAP, ABC and the PAP immunodetection systems.
•Journal of Clinical Pathology 50: 153-156. gestational endometrium using the APAAP, ABC and the PAP immunodetection systems. Journal of Clinical Pathology 50: 153-156.
•Kashima K, Yokoyama S, Tsutomu Da, Nakayama I, Nickerson PA, Noguchi S 1997. Cytoplasmic biotin-like activity interferes with immunohistochemical analysis of thyroid lesions: a comparison of antigen retrieval methods. Modern Pathology 10: 515-519.
•Shigeo Y, Kenji K, Souichi I, Daa T, Nakamaya I, Moriushi A 1993. Biotin-containing intranuclear inclusions in endometrial glands during gestation and puerperium. American Journal of Clinical Pathology 99: 13-17.
•Sickel JZ, Di Sant' Agnese A 1994. Anomalous immunostaining of `optically clear' nuclei in gestational endometrium. Archives of Pathology and Laboratory Medicine 118: 831-833.
Bibliografía
Manual of diagnostic antibodies for immunohistology / Anthony S.-Y. Leong, Kumarasen Cooper, F. Joel W.-M. Leong.
