Sources/Clones
Becton Dickinson (Leu 9), Biodesign (WT1, WM31, 8H8.1), Biogenesis (WM31), Coulter (3A1), Cymbus Bioscience (WM31), Dako (DK24), GenTrak, Immunotech, Oncogene U3A1E), Sanbio (WT1), Seralab and Serotec (B-F12, B-5, HNE51).
Fixation/Preparation
Fresh frozen-tissue and fresh cytologic preparations.
Background
CD 7 antigen is a cell surface glycoprotein of 40 kD expressed on the surface of immature and mature T-cells and natural killer cells. It is a member of the immunoglobulin gene superfamily and is the first T-cell lineage-associated antigen to appear in T-cell ontogeny, being expressed in prethymic T-cell precursors (preceding CD 2 expression) and in myeloid precursors in fetal liver and bone marrow and persisting in circulating T-cells. While its precise function is not known, there is a recent suggestion that the molecule functions as an Fc receptor for IgM (Lazarovits et al, 1994).
Applications
CD 7 is the most consistently expressed T-cell antigen in lymphoblastic lymphomas and leukemias and is specific for T-cell lineage and is therefore a useful marker in the identification of such neoplastic proliferations. In mature postthymic T-cell neoplasms, it is the most common pan-T antigen to be aberrantly absent and its absence in a T-cell population is a useful pointer to a neoplastic conversion.
Comments
Current antibodies are not immunoreactive in fixed tissues.
References
•Lazarovits AI, Osman N, Le Feuvre CE et al 1994. CD7 is associated with CD3 and CD 45 in human T cells. Journal of Immunology 153: 3956-3966.
Bibliografía
Manual of diagnostic antibodies for immunohistology / Anthony S.-Y. Leong, Kumarasen Cooper, F. Joel W.-M. Leong.
Becton Dickinson (Leu 9), Biodesign (WT1, WM31, 8H8.1), Biogenesis (WM31), Coulter (3A1), Cymbus Bioscience (WM31), Dako (DK24), GenTrak, Immunotech, Oncogene U3A1E), Sanbio (WT1), Seralab and Serotec (B-F12, B-5, HNE51).
Fixation/Preparation
Fresh frozen-tissue and fresh cytologic preparations.
Background
CD 7 antigen is a cell surface glycoprotein of 40 kD expressed on the surface of immature and mature T-cells and natural killer cells. It is a member of the immunoglobulin gene superfamily and is the first T-cell lineage-associated antigen to appear in T-cell ontogeny, being expressed in prethymic T-cell precursors (preceding CD 2 expression) and in myeloid precursors in fetal liver and bone marrow and persisting in circulating T-cells. While its precise function is not known, there is a recent suggestion that the molecule functions as an Fc receptor for IgM (Lazarovits et al, 1994).
Applications
CD 7 is the most consistently expressed T-cell antigen in lymphoblastic lymphomas and leukemias and is specific for T-cell lineage and is therefore a useful marker in the identification of such neoplastic proliferations. In mature postthymic T-cell neoplasms, it is the most common pan-T antigen to be aberrantly absent and its absence in a T-cell population is a useful pointer to a neoplastic conversion.
Comments
Current antibodies are not immunoreactive in fixed tissues.
References
•Lazarovits AI, Osman N, Le Feuvre CE et al 1994. CD7 is associated with CD3 and CD 45 in human T cells. Journal of Immunology 153: 3956-3966.
Bibliografía
Manual of diagnostic antibodies for immunohistology / Anthony S.-Y. Leong, Kumarasen Cooper, F. Joel W.-M. Leong.
