Sources/Clones
Biodesign (51-13), Biogenesis (1G6, CP15), Biogenex (UM01), Dako (polyclonal) and Sanbio/Monosan (51-13).
Fixation/Preparation
Applicable to 10% neutral buffered formalin or Bouin's fixed tissue.
Background
Helicobacter pylori(HP) is a spiral bacillus that can colonize the human gastric mucosa and induce a specific humoral immunologic reaction in the host. Colonization of the gastric mucosa by HP is a very common finding in gastric ulcers and active chronic gastritis. HP is increasingly recognized as one of the most prevalent human pathogens worldwide and possibly plays a pathogenetic role in gastric carcinogenesis and primary gastric lymphogenesis. The details of the interaction between bacteria, epithelial cells and inflammatory cells are currently being explored. As effective specific treatment for HP-associated gastroduodenal disorders emerges, surgical pathologists are requested to identify the organism in endoscopic biopsies. Histologic identification of HP (with special staining methods) has been shown to be as accurate as microbiologic culture techniques (Hui et al, 1992; Genta et al, 1994).
The Signet rabbit anti-HP polyclonal antisera was raised againstH.pylori strain CH-20429 and detects antigens of the whole organism in formalin-fixed, paraffin-embedded, frozen and cytologic specimens.
Applications
Bacteria lying within the mucus and on the epithelial surface can be seen on sections stained with hematoxylin and eosin (H&E). However, organisms closely adherent to cells, insinuated in intercellular spaces or intimately associated with and perhaps phagocytosed by inflammatory cells are frequently difficult to identify.
There are several published special stains that demonstrate HP efficiently in the histologic sections (Genta et al, 1994). However, the use of immunohistochemical methods is highly specific and has an important role in selected situations (Cartun et al, 1991). For example, small gastric biopsies with a very low density of H.pylori, posttreatment biopsy specimens to assess therapeutic success or when abundant debris or mucus is present on gastric surface and pits, may benefit from identification ofH.pylori with immunohistochemistry.
Comments
Immunohistochemical methods for the detection ofH.pylori are highly specific and play an important role in selected situations, but cannot be advocated for the routine diagnosis ofH.pylori gastritis. HP-infected gastric tissue is recommended as positive control tissue.
References
•Cartun RW, Kryzmowski GA, Pedersen CA et al 1991. Immunocytochemical identification ofH.pylori in formalin-fixed gastric biopsies. Modern Pathology 4: 498-502.
•Genta RM, Robason GO, Graham DY 1994. Simultaneous visualization of Helicobacter pylori and gastric morphology: a new stain. Human Pathology 25: 221-226.
•Hui PK, Chan WY, Cheung PS, Chan JKC, Ng CS 1992. Pathologic changes of gastric mucosa colonized by H.pylori. Human Pathology 23: 548-556. colonized by H.pylori. Human Pathology 23: 548-556.
Bibliografía
Manual of diagnostic antibodies for immunohistology / Anthony S.-Y. Leong, Kumarasen Cooper, F. Joel W.-M. Leong.
Biodesign (51-13), Biogenesis (1G6, CP15), Biogenex (UM01), Dako (polyclonal) and Sanbio/Monosan (51-13).
Fixation/Preparation
Applicable to 10% neutral buffered formalin or Bouin's fixed tissue.
Background
Helicobacter pylori(HP) is a spiral bacillus that can colonize the human gastric mucosa and induce a specific humoral immunologic reaction in the host. Colonization of the gastric mucosa by HP is a very common finding in gastric ulcers and active chronic gastritis. HP is increasingly recognized as one of the most prevalent human pathogens worldwide and possibly plays a pathogenetic role in gastric carcinogenesis and primary gastric lymphogenesis. The details of the interaction between bacteria, epithelial cells and inflammatory cells are currently being explored. As effective specific treatment for HP-associated gastroduodenal disorders emerges, surgical pathologists are requested to identify the organism in endoscopic biopsies. Histologic identification of HP (with special staining methods) has been shown to be as accurate as microbiologic culture techniques (Hui et al, 1992; Genta et al, 1994).
The Signet rabbit anti-HP polyclonal antisera was raised againstH.pylori strain CH-20429 and detects antigens of the whole organism in formalin-fixed, paraffin-embedded, frozen and cytologic specimens.
Applications
Bacteria lying within the mucus and on the epithelial surface can be seen on sections stained with hematoxylin and eosin (H&E). However, organisms closely adherent to cells, insinuated in intercellular spaces or intimately associated with and perhaps phagocytosed by inflammatory cells are frequently difficult to identify.
There are several published special stains that demonstrate HP efficiently in the histologic sections (Genta et al, 1994). However, the use of immunohistochemical methods is highly specific and has an important role in selected situations (Cartun et al, 1991). For example, small gastric biopsies with a very low density of H.pylori, posttreatment biopsy specimens to assess therapeutic success or when abundant debris or mucus is present on gastric surface and pits, may benefit from identification ofH.pylori with immunohistochemistry.
Comments
Immunohistochemical methods for the detection ofH.pylori are highly specific and play an important role in selected situations, but cannot be advocated for the routine diagnosis ofH.pylori gastritis. HP-infected gastric tissue is recommended as positive control tissue.
References
•Cartun RW, Kryzmowski GA, Pedersen CA et al 1991. Immunocytochemical identification ofH.pylori in formalin-fixed gastric biopsies. Modern Pathology 4: 498-502.
•Genta RM, Robason GO, Graham DY 1994. Simultaneous visualization of Helicobacter pylori and gastric morphology: a new stain. Human Pathology 25: 221-226.
•Hui PK, Chan WY, Cheung PS, Chan JKC, Ng CS 1992. Pathologic changes of gastric mucosa colonized by H.pylori. Human Pathology 23: 548-556. colonized by H.pylori. Human Pathology 23: 548-556.
Bibliografía
Manual of diagnostic antibodies for immunohistology / Anthony S.-Y. Leong, Kumarasen Cooper, F. Joel W.-M. Leong.
