Sources/Clones
Accurate (T2G1), American Diagnostica (3B622, 352, 350), Biodesign (polyclonal), Biogenesis (2F7) and Serotec (E8).
Fixation/Preparation
The antigen is resistant to formalin fixation and proteolytic digestion or HIER enhances immunoreactivity.
Background
Proteolytic conversion of fibrinogen to fibrin results in selfassembly to form a clot matrix that subsequently becomes crosslinked by factor XIIIa to form the main structural element of the thrombus in vivo. The roles of fibrin and its precursor have been extensively studied both in vitro and in vivo (Lorand, 1965 Mosessan, 1992, 1997; Blomback, 1994, 1996; Gaffney, 1997).
Applications
Diagnostic applications of fibrin are mainly limited to the study of glomerulopathy (Dowling, 1993) with sporadic use of antifibrin to identify fibrin deposits and thrombi in extrarenal sites (Bini & Kudryk, 1994; Takahashi et al, 1996; Imokawa et al, 1997).
Comments
The diagnostic applications of antifibrin are limited to specific situations.
References
•Bini A, Kudryk BJ 1994. Fibrinogen and fibrin in the arterial wall. Thrombosis Research 75: 337-341.
•Blomback B 1994. Fibrinogen structure, activation and polymerization and fibrin gel structure. Thrombosis Research 75: 327-328.
•Blomback B 1996. Fibrinogen and fibrin-proteins with complex roles in hemostasis and thrombosis. Thrombosis Research 83: 1-75.
•Dowling JP 1993. Immunohistochemistry of renal diseases and tumours. In: Leong AS-Y (ed) Applied immunohistochemistry for surgical pathologists. London: Edward Arnold, pp 210-259.
•Gaffney PJ 1997. Structure of fibrinogen and degradation products of fibrinogen and fibrin. British Medical Bulletin 33: 245-251.
•Imokawa S, Sato A, Hayakawa H et al 1997. Tissue factor expression and fibrin deposition in the lungs of patients with idiopathic pulmonary fibrosis and systemic sclerosis. American Journal of Respiratory and Critical Care Medicine 156 (2 pt 1): 631-636.
•Lorand L 1965. Physiological roles of fibrinogen and fibrin. Federation Proceedings 24: 784-793.
•Mosessan MW 1992. The roles of fibrinogen and fibrin in hemostasis and thrombosis. Seminars in Hematology 29: 177-188.
•Mosessan MW 1997. Fibrinogen and fibrin polymerization: appraisal of the binding events that accompany fibrin generation and fibrin clot assembly. Blood Coagulation and Fibrinolysis 8: 257-267. accompany fibrin generation and fibrin clot assembly. Blood Coagulation and Fibrinolysis 8: 257-267.
•Takahashi H, Shibata Y, Fujita S, Okabe H 1996. Immunohistochemical findings of arterial fibrinoid necrosis in major and lingual minor salivary glands of primary Sjogren's syndrome. Anatomical and Cellular Pathology 12: 145-157.
Bibliografía
Manual of diagnostic antibodies for immunohistology / Anthony S.-Y. Leong, Kumarasen Cooper, F. Joel W.-M. Leong.
Accurate (T2G1), American Diagnostica (3B622, 352, 350), Biodesign (polyclonal), Biogenesis (2F7) and Serotec (E8).
Fixation/Preparation
The antigen is resistant to formalin fixation and proteolytic digestion or HIER enhances immunoreactivity.
Background
Proteolytic conversion of fibrinogen to fibrin results in selfassembly to form a clot matrix that subsequently becomes crosslinked by factor XIIIa to form the main structural element of the thrombus in vivo. The roles of fibrin and its precursor have been extensively studied both in vitro and in vivo (Lorand, 1965 Mosessan, 1992, 1997; Blomback, 1994, 1996; Gaffney, 1997).
Applications
Diagnostic applications of fibrin are mainly limited to the study of glomerulopathy (Dowling, 1993) with sporadic use of antifibrin to identify fibrin deposits and thrombi in extrarenal sites (Bini & Kudryk, 1994; Takahashi et al, 1996; Imokawa et al, 1997).
Comments
The diagnostic applications of antifibrin are limited to specific situations.
References
•Bini A, Kudryk BJ 1994. Fibrinogen and fibrin in the arterial wall. Thrombosis Research 75: 337-341.
•Blomback B 1994. Fibrinogen structure, activation and polymerization and fibrin gel structure. Thrombosis Research 75: 327-328.
•Blomback B 1996. Fibrinogen and fibrin-proteins with complex roles in hemostasis and thrombosis. Thrombosis Research 83: 1-75.
•Dowling JP 1993. Immunohistochemistry of renal diseases and tumours. In: Leong AS-Y (ed) Applied immunohistochemistry for surgical pathologists. London: Edward Arnold, pp 210-259.
•Gaffney PJ 1997. Structure of fibrinogen and degradation products of fibrinogen and fibrin. British Medical Bulletin 33: 245-251.
•Imokawa S, Sato A, Hayakawa H et al 1997. Tissue factor expression and fibrin deposition in the lungs of patients with idiopathic pulmonary fibrosis and systemic sclerosis. American Journal of Respiratory and Critical Care Medicine 156 (2 pt 1): 631-636.
•Lorand L 1965. Physiological roles of fibrinogen and fibrin. Federation Proceedings 24: 784-793.
•Mosessan MW 1992. The roles of fibrinogen and fibrin in hemostasis and thrombosis. Seminars in Hematology 29: 177-188.
•Mosessan MW 1997. Fibrinogen and fibrin polymerization: appraisal of the binding events that accompany fibrin generation and fibrin clot assembly. Blood Coagulation and Fibrinolysis 8: 257-267. accompany fibrin generation and fibrin clot assembly. Blood Coagulation and Fibrinolysis 8: 257-267.
•Takahashi H, Shibata Y, Fujita S, Okabe H 1996. Immunohistochemical findings of arterial fibrinoid necrosis in major and lingual minor salivary glands of primary Sjogren's syndrome. Anatomical and Cellular Pathology 12: 145-157.
Bibliografía
Manual of diagnostic antibodies for immunohistology / Anthony S.-Y. Leong, Kumarasen Cooper, F. Joel W.-M. Leong.
