Sources/Clones
Dako (VS38c).
Fixation/Preparation
The antibody is reactive in paraffin-embedded sections and staining is enhanced by heat-induced antigen retrieval.
Background
VS38 was shown to detect a protein similar to the p63 protein. The latter is a non-glycated, reversibly palmitoylated type II transmembrane protein, which is found in rough endoplasmic reticulum. VS38 was originally described as a marker of neoplastic and non-neoplastic plasma cells (Turley et al, 1994).
Applications
It is now recognized that the protein detected by VS38 is not exclusive to plasma cells but serves to distinguish plasma cells from other lymphoid cells because of their high secretory activity (Banham et al, 1997). It has been recommended for inclusion in a panel of antibodies for the immunostaining of bone marrow trephines fixed in common fixatives including Bouin's solution (Gala et al, 1997).
VS38 immunostaining has been reported in neuroendocrine tumors and in melanocytic lesions and caution should be exercised when using this marker to identify plasma cell lineage (Banarjee et al, 1997).
Comments
There is a need for a specific marker of plasma cell differentiation as a variety of neoplastic cells can display plasmacytoid features and the converse, that is, poorly differentiated plasma cells and plasmacytoid cells can be difficult to recognize morphologically. In a recent study of endometritis, it was found that besides labeling plasma cells, VS38 also stained epithelium and stromal cells of the endometrium. In contrast, CD 38 produced strong labeling of plasma cells and not the other endometrial components, suggesting that CD 38 may be a more specific marker of plasma cell differentiation (Leong et al, 1997).
References
•Banerjee SS, Shanks JH, Hasleton PS 1997. VS38 immunostaining in neuroendocrine tumors. Histopathology 30:2 56-259.
•Banham AH, Turley H, Pulford K, et al 1997. The plasma cell associated antigen detectable by antibody VS38 is the p63 rough endoplasmic reticulum protein. Journal of Clinical Pathology 50: 485-489.
•Gala JL, Chenut F, Hong KB, et al 1997. A panel of antibodies for the immunostaining of Bouin's fixed bone marrow trephine biopsies. Journal of Clinical Pathology 50: 521-524.
•Leong AS-Y, Vinyuvat S, Leong FJWM, Suthipintawong C 1997. Anti-CD38 and VS38 antibodies for the detection of plasma cells in the diagnosis of chronic endometritis. Applied Immunohistochemistry 5: 189-193.
•Turley H, Jones M, Erber W, et al 1994. VS38: a new monoclonal antibody for detecting plasma cell differentiation in routine sections. Journal of Clinical Pathology 47: 418-422.
Bibliografia
Manual of diagnostic antibodies for immunohistology / Anthony S.-Y. Leong, Kumarasen Cooper, F. Joel W.-M. Leong.
Dako (VS38c).
Fixation/Preparation
The antibody is reactive in paraffin-embedded sections and staining is enhanced by heat-induced antigen retrieval.
Background
VS38 was shown to detect a protein similar to the p63 protein. The latter is a non-glycated, reversibly palmitoylated type II transmembrane protein, which is found in rough endoplasmic reticulum. VS38 was originally described as a marker of neoplastic and non-neoplastic plasma cells (Turley et al, 1994).
Applications
It is now recognized that the protein detected by VS38 is not exclusive to plasma cells but serves to distinguish plasma cells from other lymphoid cells because of their high secretory activity (Banham et al, 1997). It has been recommended for inclusion in a panel of antibodies for the immunostaining of bone marrow trephines fixed in common fixatives including Bouin's solution (Gala et al, 1997).
VS38 immunostaining has been reported in neuroendocrine tumors and in melanocytic lesions and caution should be exercised when using this marker to identify plasma cell lineage (Banarjee et al, 1997).
Comments
There is a need for a specific marker of plasma cell differentiation as a variety of neoplastic cells can display plasmacytoid features and the converse, that is, poorly differentiated plasma cells and plasmacytoid cells can be difficult to recognize morphologically. In a recent study of endometritis, it was found that besides labeling plasma cells, VS38 also stained epithelium and stromal cells of the endometrium. In contrast, CD 38 produced strong labeling of plasma cells and not the other endometrial components, suggesting that CD 38 may be a more specific marker of plasma cell differentiation (Leong et al, 1997).
References
•Banerjee SS, Shanks JH, Hasleton PS 1997. VS38 immunostaining in neuroendocrine tumors. Histopathology 30:2 56-259.
•Banham AH, Turley H, Pulford K, et al 1997. The plasma cell associated antigen detectable by antibody VS38 is the p63 rough endoplasmic reticulum protein. Journal of Clinical Pathology 50: 485-489.
•Gala JL, Chenut F, Hong KB, et al 1997. A panel of antibodies for the immunostaining of Bouin's fixed bone marrow trephine biopsies. Journal of Clinical Pathology 50: 521-524.
•Leong AS-Y, Vinyuvat S, Leong FJWM, Suthipintawong C 1997. Anti-CD38 and VS38 antibodies for the detection of plasma cells in the diagnosis of chronic endometritis. Applied Immunohistochemistry 5: 189-193.
•Turley H, Jones M, Erber W, et al 1994. VS38: a new monoclonal antibody for detecting plasma cell differentiation in routine sections. Journal of Clinical Pathology 47: 418-422.
Bibliografia
Manual of diagnostic antibodies for immunohistology / Anthony S.-Y. Leong, Kumarasen Cooper, F. Joel W.-M. Leong.
