Source
Dako (UEA-1).
Fixation/Preparation
Carbohydrates reactive with UEA-1 are generally active in formalin-fixed, paraffin-embedded tissue sections. Background staining may be reduced by the addition of 5% human serum to the anti-UEA-1 dilution buffer.
Background
UEA-1 is a plant lectin isolated fromUlex europaeus seeds (gorse seeds) by affinity chromatography. The lectin is homogeneous, containing 4.2% neutral sugar and 1.4% glucosamine (Horejsi, 1979). Its molecular weight is approximately 110 kD, comprising two covalently bound basic subunits. UEA-1 is specific to certain terminala-L-fucosyl residues of glycoconjugates and also detects blood group H antigen (Pereira et al, 1978).
Applications
UEA-1 has been used successfully as a marker for endothelial cells. It has been shown to be more sensitive for benign vascular tumors than thrombomodulin or factor VIII-related antigen (Yonezawa et al, 1987). In fact, other workers have shown UEA-1 to be a more sensitive marker for endothelial cells of vascular tumors than factor VIII-related antigen (Miettinen et al, 1983; Ordonez & Batsakis, 1984). UEA-1 does not distinguish between the endothelial cells of blood vessels and lymphatics (Suthipintawong et al, 1995). In some tissues Ulex lectin has demonstrated additional binding to epithelial structures (Holthofer et al, 1982). This latter immunoreaction has been exploited with UEA-1 demonstrating specific binding to collecting duct carcinoma of the kidney, enabling distinction from other types of renal cell carcinoma (Amin et al, 1997).
Comments
There are more specific and sensitive markers of endothelial cells available, such as C 34 and CD 31. It would appear that the use of UEA-1 is confined to identifying collecting duct carcinoma of the kidney. Benign vascular tissue makes appropriate positive controls for UEA-1.
References
•Amin MB, Varma MD, Tickoo SK, Ro JY 1997 Collecting duct carcinoma of the kidney. Advances in Anatomic Pathology 4: 85-94.
•Holthofer H, Virtanen I, Kariniemi AL, Hormia M, Linder E, Miettinen A 1982 Ulex europaeus I lectin as a marker for vascular endothelium in human tissues. Laboratory Investigation 47: 60-66.
•Horejsi V 1979 Properties of Ulex europaeus II lectin isolated by affinity chromatography. Biochima Biophysiologia Acta 577: 389-393.
•Miettinen M, Holthofer H, Lehto VP, Miettinen A, Virtanen I 1983 Ulex europaeus I lectin as a marker for tumors derived from endothelial cells. American Journal of Clinical Pathology 79: 32-36.
•Ordonez NG, Batsakis JG 1984 Comparison of Ulex europaeus I lectin and Factor VIII-related antigen in vascular lesions. Archives of Pathology and Laboratory Medicine 108: 129-132.
•Pereira ME, Kisailus EC, Gruezo F, Kabat EA 1978 Immunohistochemical studies on the combining site of the blood group H-specific lectin 1 from Ulex europeus seeds. Archives of Biochemistry and Biophysiology 185: 108-115. site of the blood group H-specific lectin 1 from Ulex europeus seeds. Archives of Biochemistry and Biophysiology 185: 108-115.
•Suthipintawong C, Leong AS-Y, Vinyuvat S 1995 A comparative study of markers for lymphangiomas and hemangiomas. Applied Immunohistochemistry 3: 239-244.
•Yonezawa S, Maruyama I, Sakae K et al 1987 Thrombomodulin as a marker for vascular tumors. Comparative study with Factor VIII and Ulex europaeus I lectin. American Journal of Clinical Pathology 88: 405-411
Bibliografia
Manual of diagnostic antibodies for immunohistology / Anthony S.-Y. Leong, Kumarasen Cooper, F. Joel W.-M. Leong.
Dako (UEA-1).
Fixation/Preparation
Carbohydrates reactive with UEA-1 are generally active in formalin-fixed, paraffin-embedded tissue sections. Background staining may be reduced by the addition of 5% human serum to the anti-UEA-1 dilution buffer.
Background
UEA-1 is a plant lectin isolated fromUlex europaeus seeds (gorse seeds) by affinity chromatography. The lectin is homogeneous, containing 4.2% neutral sugar and 1.4% glucosamine (Horejsi, 1979). Its molecular weight is approximately 110 kD, comprising two covalently bound basic subunits. UEA-1 is specific to certain terminala-L-fucosyl residues of glycoconjugates and also detects blood group H antigen (Pereira et al, 1978).
Applications
UEA-1 has been used successfully as a marker for endothelial cells. It has been shown to be more sensitive for benign vascular tumors than thrombomodulin or factor VIII-related antigen (Yonezawa et al, 1987). In fact, other workers have shown UEA-1 to be a more sensitive marker for endothelial cells of vascular tumors than factor VIII-related antigen (Miettinen et al, 1983; Ordonez & Batsakis, 1984). UEA-1 does not distinguish between the endothelial cells of blood vessels and lymphatics (Suthipintawong et al, 1995). In some tissues Ulex lectin has demonstrated additional binding to epithelial structures (Holthofer et al, 1982). This latter immunoreaction has been exploited with UEA-1 demonstrating specific binding to collecting duct carcinoma of the kidney, enabling distinction from other types of renal cell carcinoma (Amin et al, 1997).
Comments
There are more specific and sensitive markers of endothelial cells available, such as C 34 and CD 31. It would appear that the use of UEA-1 is confined to identifying collecting duct carcinoma of the kidney. Benign vascular tissue makes appropriate positive controls for UEA-1.
References
•Amin MB, Varma MD, Tickoo SK, Ro JY 1997 Collecting duct carcinoma of the kidney. Advances in Anatomic Pathology 4: 85-94.
•Holthofer H, Virtanen I, Kariniemi AL, Hormia M, Linder E, Miettinen A 1982 Ulex europaeus I lectin as a marker for vascular endothelium in human tissues. Laboratory Investigation 47: 60-66.
•Horejsi V 1979 Properties of Ulex europaeus II lectin isolated by affinity chromatography. Biochima Biophysiologia Acta 577: 389-393.
•Miettinen M, Holthofer H, Lehto VP, Miettinen A, Virtanen I 1983 Ulex europaeus I lectin as a marker for tumors derived from endothelial cells. American Journal of Clinical Pathology 79: 32-36.
•Ordonez NG, Batsakis JG 1984 Comparison of Ulex europaeus I lectin and Factor VIII-related antigen in vascular lesions. Archives of Pathology and Laboratory Medicine 108: 129-132.
•Pereira ME, Kisailus EC, Gruezo F, Kabat EA 1978 Immunohistochemical studies on the combining site of the blood group H-specific lectin 1 from Ulex europeus seeds. Archives of Biochemistry and Biophysiology 185: 108-115. site of the blood group H-specific lectin 1 from Ulex europeus seeds. Archives of Biochemistry and Biophysiology 185: 108-115.
•Suthipintawong C, Leong AS-Y, Vinyuvat S 1995 A comparative study of markers for lymphangiomas and hemangiomas. Applied Immunohistochemistry 3: 239-244.
•Yonezawa S, Maruyama I, Sakae K et al 1987 Thrombomodulin as a marker for vascular tumors. Comparative study with Factor VIII and Ulex europaeus I lectin. American Journal of Clinical Pathology 88: 405-411
Bibliografia
Manual of diagnostic antibodies for immunohistology / Anthony S.-Y. Leong, Kumarasen Cooper, F. Joel W.-M. Leong.
