Sources/Clones
Biodesign (8E6, polyclonal), Biogenesis (polyclonal), Calbiochem/Novocastra (monoclonal), Dako
(polyclonal), and Fitzgerald (polyclonal).
Fixation/Preparation
The antibodies are immunoreactive in fixed, paraffin-embedded tissues. Immunoreactivity is increased following proteolytic digestion.
Background
a-1-Antichymotrypsin (AACT), a 68 kD glycoprotein, is a serum protease inhibitor which is
synthesized mainly by cells of the mononuclear phagocytic system. AACT was initially employed as a marker of histiocytes (monocytes/macrophages) but the demonstration of this enzyme in a large variety of normal and neoplastic tissues of both epithelial and mesenchymal derivation has resulted in only restricted use in diagnostic immunohistochemistry. It most likely identifies cells that are rich in phagolysosomes and has no tissue specificity. With restricted settings, AACT can be of value in diagnostic immunohistology. (See discussion on a-1-antitrypsin.)
Bibliografia
Manual of diagnostic antibodies for immunohistology / Anthony S.-Y. Leong, Kumarasen Cooper, F. Joel W.-M. Leong.
Biodesign (8E6, polyclonal), Biogenesis (polyclonal), Calbiochem/Novocastra (monoclonal), Dako
(polyclonal), and Fitzgerald (polyclonal).
Fixation/Preparation
The antibodies are immunoreactive in fixed, paraffin-embedded tissues. Immunoreactivity is increased following proteolytic digestion.
Background
a-1-Antichymotrypsin (AACT), a 68 kD glycoprotein, is a serum protease inhibitor which is
synthesized mainly by cells of the mononuclear phagocytic system. AACT was initially employed as a marker of histiocytes (monocytes/macrophages) but the demonstration of this enzyme in a large variety of normal and neoplastic tissues of both epithelial and mesenchymal derivation has resulted in only restricted use in diagnostic immunohistochemistry. It most likely identifies cells that are rich in phagolysosomes and has no tissue specificity. With restricted settings, AACT can be of value in diagnostic immunohistology. (See discussion on a-1-antitrypsin.)
Bibliografia
Manual of diagnostic antibodies for immunohistology / Anthony S.-Y. Leong, Kumarasen Cooper, F. Joel W.-M. Leong.
